![]() All of this comes at minimal costs (in fact, in many ways it is cheaper than making standard TruSeq libraries), but with all the analytical benefits. ![]() Using haplotagging, we can map genes, detect signatures of selection, track the breakdown of haplotypes, and also detect large structural rearrangements like inversions. This allows us to create genomic data of a scale and quality unlike anytime before. Because it is now a 10-minute enzymatic reaction with PCR, we have now made thousands of haplotagged libraries from humans, mice, butterflies and more organisms. We have now shown, in Meier et al., PNAS, 2021, that it works! Not only can we make linked-read libraries and phase variants into megabase-long phased blocks in single individiuals just like 10X's Chromium platform. Unlike 10X Genomics's Chromium platform, which isolates and manipulates individual DNA fragments ("molecules") in microfluidic droplets, CPTseq takes advantage of Tn5, a transposase protein that is capable of transferring moleuclar adapters to DNA sequences in a single enzymatic reaction. They show a completely different way of molecular barcoding from the leading commercial method by 10X Genomics at the time. Solution: haplotagging - a fast and easy technique for linked-read sequencingĪ couple of papers on continguity-preserving tagmentation sequencing (CPTseq) from Jay Shendure (UW Seattle) and Frank Steemers (Illumina) provided the answer (Amini et al., Nat Biotech, 2014 Zhang et al., Nat Biotech, 2017). Long-read sequencing tend to be expensive, labourious, and error prone.Then there is linked-read sequencing, which combines the advantage of ease and accuracy of short-read sequencing with the haplotype information of long-read sequencing.īut existing commerical options are very expensive and very low throughput. We needed a new way forward. But they break up the genome into tiny pieces and so leave out haplotype information. Short-read Illumina sequencing is cheap and higly accurate. ![]() We realized that existing sequencing techniques all leave us wanting.
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